Publication Laka-library:
Genomic instability in human osteoblast cells after exposure to depleted uranium
Author | Miller, Brooks |
6-05-0-00-31.pdf | |
Date | February 2002 |
Classification | 6.05.0.00/31 (DEPLETED URANIUM - GENERAL (F.I. HEALTH CONSEQUENCES)) |
Front |
From the publication:
Journal of Environmental Radioactivity 64 (2003) 247–259 www.elsevier.com/locate/jenvrad Genomic instability in human osteoblast cells after exposure to depleted uranium: delayed lethality and micronuclei formation Alexandra C. Miller a,∗, Kia Brooks a, Michael Stewart a, Blake Anderson a, Lin Shi b, David McClain a, Natalie Page b a Applied Cellular Radiobiology Department Armed Forces Radiobiology Research Institute, Bethesda, MD 20889-5603 USA b Molecular Oncology Branch, Division of Cancer Treatment, National Cancer Institute, National Institute Health, Bethesda, MD 20892 USA Received 30 June 2001; accepted 27 February 2002 Abstract It is known that radiation can induce a transmissible persistent destabilization of the genome. We have established an in vitro cellular model using HOS cells to investigate whether genomic instability plays a role in depleted uranium (DU)-induced effects. Transmissible genomic insta- bility, manifested in the progeny of cells exposed to ionizing radiation, has been characterized by de novo chromosomal aberrations, gene mutations, and an enhanced death rate. Cell lethal- ity and micronuclei formation were measured at various times after exposure to DU, Ni, or gamma radiation. Following a prompt, concentration-dependent acute response for both end- points, there was de novo genomic instability in progeny cells. Delayed reproductive death was observed for many generations (36 days, 30 population doublings) following exposure to DU, Ni, or gamma radiation. While DU stimulated delayed production of micronuclei up to 36 days after exposure, levels in cells exposed to gamma-radiation or Ni returned to normal after 12 days. There was also a persistent increase in micronuclei in all clones isolated from cells that had bee